剪切速率对高浓度再生丝素水溶液构象的影响

对高浓度的再生丝素水溶液（15％～37％）在不同剪切速率下（1000s-1和2000s-1）的构象变化进行了研究，对剪切前后的溶液进行了DSC和Raman对比测试。发现经过剪切，丝素溶液更客易发生从无规线团或α-螺旋向β-折叠构象的转变，且转变的程度随所受剪切速率的增加而提高。而且当丝素浓度低于27％时对剪切不敏感；超过30％时则对剪切敏感。     

再生丝素固体的微细结构

研究了蚕丝丝素水溶液经不同方式干燥后，所得到的再生丝素固体的微细结构，以及存放过程中丝素的结构变化，指出经30℃热风干燥或经高于－20℃冷冻干燥后，所得到的丝素固体的结构是无定形和Silk I共存，在室内温度湿度环境中存放后，部分无定形结构转化为Silk I；蚕丝丝素水溶液经－80－－20℃冷冻干燥后，所得到的丝素膜的结构主要是无定形，含少量Silk II，存放过程中部分无定形结构转化为SilkI；室温放所自然形成的丝素凝胶中，丝素的结构是无定形和Silk Ⅱ共存；丝素水溶液表面膜中甘氨酸和丙氨酸的含量较高，表面膜的结构主要是Silk I和Tilk Ⅱ结晶度高。     

罗平布依文化的内涵与价值建构

罗平布依化是我国布依族化的重要组成部分。它既保持着布依整体原生化的优秀内质，又有独特的再生创造话性。其特点是：(1)以美丽的自然环境为裁体；(2)以独特的民俗民趣为形态；(3)以鲜明的民族气质为核心；(4)以丰富的民间艺术形式为见证。强化这些特点，是建构罗平布依化价值的基本思路。“民族尊重”和“宽容姿态”是罗平布依化价值建构的起点和最后归宿。     

Cu（Ⅱ） effect on the conformation of regenerated silk fibroin in dilute aqueous solution

Much attention has been paid to the natural mechanism of silkworm spinning due to the impressive mechanical properties of the natural fibers. In this work, we studied the effect of Cu（Ⅱ） ions on the secondary structure of Bombyx mori regenerated silk fibroin （SF） in dilute solution by circular dichroism （CD）. The results indicate that a given amount of Cu（Ⅱ） induces the SF conformational transition from random coil to β-sheet, however, further addition of Cu（Ⅱ） is unfavorable for this conversion. Meanwhile, the conformational changes induced by Cu（Ⅱ） follow a nucleation-dependent aggregation mechanism, which is similar to that found in Prion protein （PrP） denaturation and Aβ-peptide aggregations, leading to the neurodegenerative disease. This work would help one understand further the natural spinning process of silkworm. Additionally, it would be significant for the study of the nervous system diseases, because silk fibroin, extracted in large amounts from Bombyx mori silkworm gland, could be a proper model to study PrP denaturation and Aβ-peptide aggregations.     

再生丝心蛋白和羧甲基纤维素复合膜的特性及其在有机相葡萄糖生物传感器中的应用

用再生丝心蛋白和羧甲基纤维素构成的复合膜作为固定葡萄糖氧化酶的基质,并用红外光谱测试了该复合膜的结构,用扫描电子显微镜观察了其形态．用上述复合膜材料固定葡萄糖氧化酶和羧酸二茂铁（或７,７,８,８－四氰代二甲基苯醌,ＴＣＮＱ）制成的葡萄糖生物传感器具有较好的灵敏度与稳定性     

再生柞蚕丝素蛋白膜的制备及其结构研究

用硫氰酸锂溶液溶解柞蚕丝素纤维，制备再生柞蚕丝素膜。用物理化学方法研究再生柞蚕丝素蛋白膜的结构特征以及暴露在空气中所发生的结构变化。指出再生柞蚕丝素膜内丝素蛋白的分子构象为α-螺旋和无规线团结构共存。空气环境存放30d后，分子构象只会发生无规线团向α-螺旋结构的少量转化，而不会向β-折叠结构转化。     

丝蛋白膜的加工工艺和应用概述

探讨了丝素蛋白膜加工工艺及影响因素，并概述了其主要应用。     

生物除磷脱氮工艺再生回用水作为冷却水应注意的问题

污水处理的再生水回用与冷却用水采用除磷脱氮工艺,是把好水质质量关的重要保证。提出在生物除磷脱氮工艺的管理运行中,要采用科学方法,使水处理工艺达到最高能效;在运行管理中要掌握原理,注意生物除磷脱氮的主要影响因素和其他方面的问题。     

遗留资源再回收顶板运动及压力显现特征

为分析遗留下分层开采时再生顶板的运动规律及矿压显现特征,以北皂煤矿油页岩分层下行开采为背景,结合前期研究中建立的下分层再生顶板碎裂结构模型及其分带特征,通过改进传统的相似材料配比构建了下分层再生顶板碎裂结构相似材料试验模型,并对下分层开采中的顶板运动、支承压力分布及支架受力特点进行了研究.结果表明:受二次采动影响,再生顶板垮落岩块碎胀系数较小,压实程度较好.上、下分层采高接近情况下,下分层工作面支承压力影响范围及峰值均小于上分层,分别为上分层的38%和51%;下分层支架受力为上分层的91.6%;下分层采出后顶板中不规则冒落带及规则冒落带高度均增加至采前的3倍;裂隙带高度增加为采前的1.6倍.为类似条件的下分层采场设计、支架选型提供依据.     

Tussah Silk Fibroin Porous Scaffolds Prepared with a Mild Self-assembly Process for Controlled Drug Release

Besides excellent biodegradability and biocompatibility,a useful tissue engineering scaffold should provide favorable surface properties,outstanding mechanical strength and controlled drug release property. In this paper,a mild process to prepare porous tussah silk fibroin（ TSF） scaffolds from aqueous solution was described. The n-butanol was used to control the self-assembly of tussah silk. The scaffolds with different TSF concentrations and the same volume showed differences in pore size and distribution. The maximum porosity of the poprepared porous scaffolds was 80% in this paper. And the pore size of the prepared porous scaffolds with different concentrations was between 10μm and 230 μm. X-ray diffraction（ XRD） analysis revealed that amorphous TSF was crystallized to β-sheet secondary structure upon gelatin. The TSF scaffolds for controlled drug release was studied and the result showed that the time of drug release was significantly longer. The produced TSF scaffolds with sustained drug release have potential application in tissue engineering.